Developed at the Max-Planck-Institute of Biochemistry in Martinsried the Sonation column oven is used for the temperature regulation of the column in ESI-Nanospray applications. Due to a constant column temperature the results are getting more precise and with a higher column temperature longer columns and faster washing cycles can be achieved.
The Sonation column oven has been developed with the goal to solve three problems in the ESI-Nanospray workflow:
- The influence of a changing room temperature.
In most labs the room temperature is not constant. Air conditions or opened doors are causing a changing temperature that influences the results.
- The length of the column is limited due to the maximum pressure of the used HPLC.
The longer the used column, the higher the pressure must be to maintain the same flowrate.
- The washout of the columns consumes a lot of time.
Even with the maximum pressure of the HPLC the washing cycles needs a lot of time.
With the Sonation column oven the temperature of the column is now completely independent from the room temperature. The column can also be heated to high temperatures to accelerate the washout cycles or getting higher flowrates during the run. Also the use of longer columns is now possible.
With the software that comes with the oven the temperature can be adjusted direct on the PC. It can also be switched between two temperatures automatically using an instrument method in Xcalibur.
The Sonation column oven allows for shortening or
lengthening of gradients without loss of sensitivity
Dr. Richard Scheltema (MPI of biochemistry Martinsried, Germany)
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